Abstract
Abstract Liquid chromatogrophic characteristics of twelve selected dopamine-derived tetrahydroisoquinoline and tetrahydroprotoberberine alkaloids on commercially available 10 μ cation-exchange columns (Partisil 10 SCX, Vydac TP 401 SCX and Nucleosil SA) were examined. Effects of mobile phase ionic strength, pH, addition of organic modifiers, and column temperature on retention and efficiency were determined. Capacity factors (k') were linear with respect to 1/ionic strength for Nucleosil and Vydac, but not for Partisil columns. Retention of compounds varied as follows: Nucleosil > Vydac > Partisil. Mobile phase pH had little effect on retention or selectivity. However, chromatographic efficiency was adversely affected by mobile phase pH greater than 5.5. Organic modifiers decreased elution time in the following order: 1-butanol > dioxane > 2-propanol ≃ acetonitrile > ethanol. Elevated column temperature decreased capacity factors and increased efficiency. Conditions for the separation and detection of low...
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