Analysis of DNA repair synthesis in peripheral blood lymphocytes from patients with well-controlled and uncontrolled asthma

Abstract

<b>Introduction:</b> The aim of the present study was to assess the intensity of the DNA repair synthesis in lymphocytes of patients with well-controlled and uncontrolled asthma. <b>Patients and Methods:</b> The levels of spontaneous and UV-induced unscheduled DNA synthesis (UDS) in peripheral blood lymphocytes were evaluated for 58 asthmatic patients aged 16-70 years. The control group consisted of 15 healthy subjects. <b>Results:</b> The intensity of spontaneous UDS in lymphocytes of asthmatic patients was established to be significantly (p &lt; 0.02) higher than that in the cells of healthy controls. The values of UV-induced UDS did not differ significantly in patients and healthy subjects (6.6 ± 0.43% and 7.4± 1.20%, respectively, p &gt; 0.05). The rates of UV-induced UDS were revealed to be significantly lower in patients with uncontrolled asthma than in those with well-controlled condition (5.9 ± 0.78% and 8.1± 0.83%, respectively, p &lt; 0.05) in allergic asthmatics and did not depend on the asthma control in patients with non-allergic phenotype (5.5 ± 0.81% and 5.6 ± 0.80%, respectively, p &gt; 0.05). The levels of spontaneous UDS in patients with uncontrolled and well-controlled asthma did not differ significantly (p &gt; 0.05) irrespective of the disease phenotype. <b>Conclusions:</b> We concluded that the intensity of spontaneous UDS is significantly higher in lymphocytes of asthmatic patients as compared with healthy subjects. The levels of UV-induced UDS in lymphocytes of allergic asthmatics were established to be significantly lower in patients with uncontrolled asthma than in those with well-controlled condition