Abstract
The topological analysis of DNA knots and catenanes produced by different enzymes acting on DNA molecules becomes possible by an electron microscopical technique in which the DNA molecules are covered with recA protein (1,2). The recA covering of the DNA increases the thickness of the filaments from 2 nm to 10 nm and by this allows to distinguish between underlying and overlying segments in knoted or catenated DNA molecules adsorbed to support films used for electron microscopy.
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