Abstract
BackgroundThe possibility of culturing Parmentiera cereifera in vitro was tested. Shoot tips and lateral buds were cultured in three media that were based on Murashige and Skoog (MS) but supplemented with different types and concentrations of growth regulators. Thirty-eight simple sequence repeat (SSR) primers were used to assess the genetic stability of the regenerated plantlets.ResultsLateral buds recorded the highest significant mean values for shoot, root length, and the number of leaves when cultured in MS + 1.2 mg/l of 6-benzylaminopurine (BAP) + 1.5 g/l of activated charcoal. Seeds were also grown in different media. The best results were obtained with MS basal medium. The resulting shoots were rooted in MS medium, with 1.5 g/l of activated charcoal. Regenerated plants were acclimatized in the greenhouse. The 38 SSR primers produced 63 scorable bands ranging from 1 to 3, with an average of 1.68 per primer. Fifty-five monomorphic bands were obtained that ranged from 0 to 3, with an average of 1.45 per primer. The coefficient of similarity matrix ranged from 0.92 to 1.0, with an average of 97.4. Dendrogram generated using the SSR data tended to group the in vitro plants with the mother plant into two major clusters. The first cluster contained 19 in vitro plants with the mother plant and consisted of 4 subgroups. The second cluster contained in vitro plants, P-15, which had the lowest genetic similarity (92%) with the mother plant.ConclusionsThe results revealed the increase in the degree of similarity between the tested plants in the SSR analyses. Therefore, micropropagation is a safe mode for multiplication of true-to-type plants of P. cereifera.
Highlights
The possibility of culturing Parmentiera cereifera in vitro was tested
Tissue culture experiments Shoot tips, lateral buds, and seeds of P. cereifera were obtained from one tree, grown in the Antoniades gardens, Smouha, Alexandria, Egypt (Fig. 1a)
The highest mean value recorded was 11.52 cm when the stable explants were cultured in Murashige and Skoog (MS) medium + 1.2 mg/l 6-BAP + 1.5 mg/l activated charcoal
Summary
The possibility of culturing Parmentiera cereifera in vitro was tested. Shoot tips and lateral buds were cultured in three media that were based on Murashige and Skoog (MS) but supplemented with different types and concentrations of growth regulators. Thirty-eight simple sequence repeat (SSR) primers were used to assess the genetic stability of the regenerated plantlets. Parmentiera cereifera (candle tree) belongs to the Bignoniaceae family and is a small tree native to Panama. The flowers are white, slightly fragrant, and emerges directly on the bark of the tree. The fruits are smooth, waxy skinned green-yellow, and resemble candles. The tree is grown as an ornamental for its flowers and unusual appearance (van Steenis 1977; Madulid 2000). Previous studies have highlighted the anticancer properties, in addition to its anti-inflammatory, cardioprotective, and antimicrobial properties by flavonoids, saponins, tannins, triterpenoids, terpenoids, and steroids in
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