Abstract

This study was carried out to develop an analysis method for the determination of anionic and nonionic surfactants. The condition for simultaneous analysis of anionic and nonionic surfactants by high-performance liquid chromatography (HPLC) equipped with an evaporated light scattering detector (ELSD) was investigated. Alkyl polyglucoside (APG) and alpha olefin sulfonate (AOS) were analyzed on a C18-boned silica column with a methanol and water gradient condition by HPLC/ELSD. Calibration curves were linear in the range of 100-500 mg/L on APG and 500- 4000 mg/L on AOS, with a coefficient of determination (r2) of 0.9999. The limits of detection (LOD) for APG and AOS were 2.29 and 16.55 mg/L, and the limits of quantification (LOQ) were 7.63 and 55.16 mg/L, respectively. The recoveries of the surfactants were 99.29% on APG and 96.11% on AOS. The established method provided acceptable precision and accuracy. Our methods would be useful for the detection of APG and AOS in dishwashing detergents.

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