Abstract
Flooding stress causes severe yield reduction in soybean worldwide. The development of stress-tolerant cultivars could be an effective measure to reduce the negative effects of flooding stress. Molecular information on the gene expression pattern of tolerant and susceptible genotypes under flooding stress could be valuable to improve the flooding tolerance in soybean. The objective of this study was to analyze the differentially expressed genes (DEGs) revealed by RNA sequencing in the soybean leaf tissues of tolerant (‘Paldalkong’ and ‘Danbaekkong’) and susceptible (‘NTS1116’) cultivars under flooding stress. Seedlings were grown in a well-watered condition up to the V1–V2 stage and flood-stressed by inundating ~ 10-cm water for 14 days. A total of 22,468 genes were differentially expressed in flood-stressed condition compared to the well-watered control condition, out of which 13,729, 13,405, and 13,160 were differentially expressed in ‘Paldalkong’, ‘Danbaekkong’, and ‘NTS1116’, respectively. A higher number of some of the flooding tolerance-related genes such as lipoxygenase, expansin, glutathione S-transferase, and sugar efflux transporter were up-regulated in the tolerant cultivars than in the susceptible cultivar. The number of some abscisic acid-related transcription factors of basic leucine zipper domain and myeloblastosis families was also higher in the tolerant cultivars than in the susceptible cultivar. The molecular information about the DEGs of tolerant and susceptible cultivars obtained in the present study could be valuable to improve the flooding tolerance in soybeans.
Highlights
Considering the multiple uses of soybean as sources of food, feed, biodiesel, and other industrial products, extensive efforts have been made to increase its yield worldwide
The results indicated that the flooding tolerance level of susceptible cultivar reduces as the flooding period extends
The RNA sequencing (RNA-Seq) expression analysis in this study revealed the differentially expressed genes (DEGs) in flood-tolerant and flood-susceptible cultivars under flooding stress conditions
Summary
Considering the multiple uses of soybean as sources of food, feed, biodiesel, and other industrial products, extensive efforts have been made to increase its yield worldwide. Soybean is an upland crop, the cultivation of soybean in converted paddy fields has been increased for a few years owing to comparative economic benefit, nutrient management, and government policy (Nishida et al 2013; Singh 2010). Soybean crop is generally sensitive to flooding stress which hampers crop growth and causes significant yield reduction (Ahmed et al 2013; Komatsu et al 2009). Flooding stress results from heavy rainfall and/or excessive irrigation coupled with poor drainage. This situation is further worsened under paddy
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