Abstract
The aim of this study was to scan through several biomaterials to find an optimal biomaterial to support the growth of cardiomyocytes. Neonatal rat cardiomyocytes were cultured on polylactide, chitosan, poly (1,8-octanediolco-citric acid), copolymer of poly(ethylene oxide terephthalate) and poly (butylene terephthalate), PuraMatrix™ and collagen. The suitability of biomaterials for cardiomyocyte culture was evaluated based on several parameters. The cells were characterized with time-lapse imaging, immunocytochemistry and LIVE/DEAD® staining. Collagen gel was the best biomaterial. It supported well the growth, survival and functionality of the cardiomyocytes. Polylactide and chitosan membranes supported the cell growth and survival, but these biomaterials were too stiff for further cardiac applications. In conclusion, collagen gel is a good biomaterial to obtain a 3D structure to model heart tissue.
Highlights
Myocardial damage due to infarction leads to the formation of a non-functional scar in the heart
Neonatal rat cardiomyocytes were cultured on polylactide, chitosan, poly (1,8-octanediolco-citric acid), copolymer of poly(ethylene oxide terephthalate) and poly, PuraMatrixTM and collagen
The current study examined the compatibility of a range of both natural and synthetic biomaterials to support cardiomyocyte culture in both 2D and 3D
Summary
Myocardial damage due to infarction leads to the formation of a non-functional scar in the heart. The use of medication can to some extent improve the function of the heart, but currently no treatments exist to repair the damaged myocardial tissue. Cell transplant studies have been performed in both animals and humans [2]. In addition to cell therapy, a 3D heart tissue model would be an optimal platform for studying the pathophysiology of different heart diseases as well as for drug discovery and toxicological testing. Such a heart tissue model would require a scaffold for structural support
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