Abstract
Abstract A fast and sensitive method for the determination of diethylenetriamine in water and soil by High Performance Liquid Chromatography (HPLC) with Fluorescence Detection is described. Diethylenetriamine is converted to its fluorescamine derivatives through pre-column derivatization and is separated by an isocratic reverse phase chromatographic system (Nucleosil C18) with acetonitrile and borate buffer at pH 8 as the mobile phase. Water samples at concentrations of diethylenetriamine from 20–100 μg/L were derivatized with fluorescamine and chromatographed directly through direct aqueous injections. Recoveries averaged 94 ± 7% for a population of nine samples. Soil samples at concentrations of diethylenetriamine from 0.24–1.0 μg/g were extracted with CaCl2 solution (2 M) followed by derivatization with fluorescamine. Derivatized soil extracts were then analyzed by HPLC. Recoveries averaged 21 ± 2% for a population of nine samples.
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