Abstract
ObjectiveThe aim of this study was to investigate the expression of cyclin-dependent kinase 1 (CDK1) in gastric cancer (GC), evaluate its relationship with the clinicopathological features and prognosis of GC, and analyze the advantage of CDK1 as a potential independent prognostic factor for GC.MethodsThe Cancer Genome Atlas (TCGA) data and corresponding clinical features of GC were collected. First, the aim gene was selected by combining five topological analysis methods, where the gene expression in paracancerous and GC tissues was analyzed by Limma package and Wilcox test. Second, the correlation between gene expression and clinical features was analyzed by logistic regression. Finally, the survival analysis was carried out by using the Kaplan–Meier. The gene prognostic value was evaluated by univariate and multivariate Cox analyses, and the gene potential biological function was explored by gene set enrichment analysis (GSEA).ResultsCDK1 was selected as one of the most important genes associated with GC. The expression level of CDK1 in GC tissues was significantly higher than that in paracancerous tissues, which was significantly correlated with pathological stage and grade. The survival rate of the CDK1 high expression group was significantly lower than that of the low expression group. CDK1 expression was significantly correlated with overall survival (OS). CDK1 expression was mainly involved in prostate cancer, small cell lung cancer, and GC and was enriched in the WNT signaling pathway and T cell receptor signaling pathway.ConclusionCDK1 may serve as an independent prognostic factor for GC. It is also expected to be a new target for molecular targeted therapy of GC.
Highlights
Gastric cancer (GC) is a very common malignant tumor, and its prognosis is relatively poor
The platform was GPL570 (Affymetrix Human Genome U133 Plus 2.0), and The Cancer Genome Atlas (TCGA) data with corresponding clinical features of GC were downloaded from TCGA database2 that contained 375 tumor tissue samples
The 92 hub genes were calculated by five topological analysis methods, and the top 10 ranked genes for each method were selected (Table 1), among which Cyclin-dependent kinase 1 (CDK1), VCAN, CCNB1, and AURKB were found in the intersection of the results
Summary
Gastric cancer (GC) is a very common malignant tumor, and its prognosis is relatively poor. CDK1 and Gastric Cancer overall survival (OS) rate was only 28.3% (Siegel and Naishadham, 2012). Many studies have focused on identifying new biomarkers for early diagnosis and prognosis prediction of GC (Verma and Sharma, 2018; Ji et al, 2019; Li et al, 2019; Ma et al, 2019; Zheng et al, 2019). It is very important to identify effective biomarkers for the diagnosis and prognosis of GC (Zou et al, 2016; Zeng et al, 2017, 2018; Zhang et al, 2017; Tang et al, 2018; Xu et al, 2018, 2019)
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