Abstract
An Fc-directed conjugate (mAb-MNP) of the anti-CD3 monoclonal antibody (mAb), Foralumab, and the surface amino groups on magnetic nanoparticles (MNPs) was prepared using the SiteClick antibody labeling kit. The conjugate was imaged by transmission electron microscopy and analyzed using energy dispersive spectrum and a nanoparticle tracking analyses. The average values of drift velocities (pix/frame) due to the Brownian motion of MNPs and mAb-MNPs in phosphate-buffered saline were 3.16/0.76 and 6.70/1.98 on the x-/yaxes, respectively. Two different hydrodynamic diameters of 180.0 nm and 258.8 nm were found for the MNPs and the mAb-MNPs with an actual size of 35 nm, respectively. The drift velocity was 2 times (2×) higher for mAb-MNPs than MNPs in proportion to the 2× higher value of the square of the hydrodynamic diameter of the conjugate relative to the MNPs as calculated by Stokes
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
