Analysis of Component and Antibacterial Activity of Ethanol Extract and Etyl Acetate Fraction of Pagoda (Clerodendrum paniculatum L.) Leaves against Pseudomonas aeruginosa and MRSA

Abstract

The current study aimed to determine chemical constituent and antibacterial activity of ethanol extract and ethyl acetate fraction of Clerodendrum paniculatum. Component of ethanol extract and ethyl acetate fraction analyzed by LC-MS and antibacterial activity to inhibit Pseudomonas aeruginosa and Meticilin Resistant Staphylococcus aureus (MRSA) use disk diffusion method. Dried powder and ethanol extract of C. paniculatum contains of flavonoid, glycoside, tannin and steroid/triterpenoid in phytochemical screening. Analysis of component by LC-MS show that ethanol extract of C. paniculatum contains of Erucamid, Cafeic acid, 7-Hidroxycoumarin dan linamarin. Ethyl acetate fraction of C. paniculatum contains of Erucamid, Apigenin, Cafeic acid, Kynurenic acid, Apigenin 7-O-glucuronide, 6-O-Methylscutellarin, Apigetrin, 4-Methoxycinnamic acid, 4-Coumaric acid, Scutellarin dan 7-Hidroxycoumarin. Inhibition value of ethanol extract of C. paniculatum against P. aeruginosa is 12.13±0.057; 10.83±0.058; 9.63±0.057; 8.3±0.058 in concentration of 300, 200, 100 and 50ppm. Inhibition value of ethyl acetate fraction of C. paniculatum against P. aeruginosa is 14.50±0.100; 13.73±0.058; 12.87± 0.057; 11.83±0.058 in concentration of 300, 200, 100 and 50 ppm. Inhibition value of ethanol extract of C. paniculatum against MRSA is 8.67±0.057; 7.80±0.100; 7.06±0.057; 6.43±0.057 in concentration of 300, 200, 100 and 50 ppm. Inhibition value of ethyl acetate fraction of C. paniculatum against MRSA is 9.77±0.057; 8.03 ±0.057; 7.33±0.057; 6.70±0.100 in concentration of 300, 200, 100 and 50ppm.