Analysis of community changes of coexisting bacteria during the growth of factory bottle cultured Hypsizygus marmoreus

Abstract

In the past, stakeholders paid more attention to the quality and growth of edible fungi. There have been few studies on whether other microorganisms exist in the sterilized media during the growth process. In this study, through periodic tracking, collection and isolation, sequencing, and data analysis, it was found that the jade mushroom (Hypsizygus marmoreus) does not exist independently during its life cycle. Ten different bacteria were isolated from five samples, and four of them did not interfere with the mycelial growth of H. marmoreus on the cultivation media. High-throughput sequencing revealed 30 phyla, 86 classes, 200 orders, 365 families, 760 genera, and 1,067 species of bacteria in 14 types of samples. A standard dataset that consisted of 2,790 amplicon sequence variants in the bacterial taxa was obtained. The species richness was the highest in the medium where the primordia were in the cultivation medium that they grew out (HDT_JZ), which exceeded that of the cultivation medium that had not yet been sterilized (HWM) and the cultivation medium at the harvest stage (HCS_JZ). Oxidative and degradative enzymes were the highest in the late post-ripened stage cultivation media (HHS-M) and HDT_JZ. These studies will help to understand changes in the biological environment of edible fungi cultivated in factories, which should help the scientists to plan better to regulate the production conditions of edible fungi.