Abstract
In this study, high performance liquid chromatography (HPLC) was used to analysis the content of citric acid and D-isoascorbic acid in beverages. The samples were separated by C18 chromatography column, 0.1 % phosphoric acid solution was used as the mobile phase with the flow rate of 0.5 mL/min, the column temperature was 35 °C, and the detection wavelength was 210 nm. The results showed that the content of citric acid and D-isoascorbic acid has a good linear correlation (r>0.99) within the range of 50 μg to 200 μg. The selectivity, recovery and precision of citric acid and D-isoascorbic acid were suitable. Meanwhile, this method could be used to detect the content of citric acid and D-isoascorbic acid in beverages.
Highlights
At present, food additives are widely used in the food industry
0.1 % phosphoric acid solution was used as the mobile phase with the flow rate of 0.5 mL/min, the column temperature was 35 °C, and the injection volume was 5 μL
The samples were separated by C18 chromatography column, 0.1 % phosphoric acid solution was used as the mobile phase with the flow rate of 0.5 mL/min, the column temperature was 35 °C, and the detection wavelength was 210 nm
Summary
Food additives are widely used in the food industry. As we all know, food additives will not cause harm to the human body within the appropriate dosage, but if the dosage exceeds the standard, they may cause various forms of toxicity [1]. Citric acid is often used as a kind of sour agent in beverages. It is has the taste of natural fruit, and has the effect of anti-oxidation. It can effectively prevent browning and stickiness on the surface of foods and play a role in color protection. This study aims to establish a high performance liquid chromatography method that can simultaneously detect citric acid and Disoascorbic acid in beverages, and this method could be used to determine the content of related substances in commercially available beverages
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