Abstract
BackgroundMicroRNAs (miRNAs) are a class of regulatory RNAs that regulate gene expression post-transcriptionally. Little, however, is known on the expression profile of circulating miRNAs in Tetralogy of Fallot (TOF) patients late after surgical repair. In this study, we aimed to identify the specific patterns of circulating miRNAs in blood of patients with repaired, non-syndromic TOF and to assess whether these specific miRNAs may be useful to differentiate patients with and without heart failure.MethodsSurePrint™ 8 × 60 K Human v16 miRNA arrays were used to determine miRNA expression profiles in 15 healthy controls and 37 patients after TOF repair of whom 3 had symptomatic right heart failure. The expression levels of selected miRNAs have been validated by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Enrichment analyses of altered miRNA expression were predicted using bioinformatic tools.ResultsCompared with healthy controls, a total of 49, 58 and 77 miRNAs were found to be significantly altered in TOF patients (TOF-all), TOF patients with (TOF-HF) and without symptomatic right heart failure (TOF-noHF) (>2.0-fold change, adjusted P < 0.05), respectively. Three miRNAs namely miR-181d-5p, miR-206 and miR-625-5p were validated by RT-qPCR in all TOF groups. The area under the receiver operating characteristic curve (AUC) for miR-181d-5p, miR-206 and miR-625-5p were 0.987, 0.993 and 0.769 in TOF-all and 0.990, 0.994 and 0.749 in TOF-noHF, respectively. Moreover, expression levels of miR-625-5p, miR-1233-3p and miR-421 were lower in TOF-HF compared to TOF-noHF (P = 0.012).ConclusionsAltered expression levels of circulating miRNAs were found in TOF patients late after surgical repair and are different to those seen in the right ventricular myocardium of infants with TOF. Expression levels of miR-421, miR-1233-3p and miR-625-5p are lower in TOF patients with symptomatic right heart failure and thus may indicate disease progression in these patients.
Highlights
MicroRNAs are a class of regulatory RNAs that regulate gene expression post-transcription‐ ally
In Tetralogy of Fallot (TOF) patients with symptomatic right heart failure, New York Heart Association (NYHA) class, right and left ventricular volumes were significantly elevated whereas measures of systolic ventricular function such as ejection fraction or velocity time integral above the aortic valve were significantly reduced
We identified an altered miRNA expression profile in TOF patients with and without symptomatic right heart failure when compared with age-matched healthy controls by microarray and further validated by quantitative reverse transcription-polymerase chain reaction (RT-qPCR) analyses
Summary
MicroRNAs (miRNAs) are a class of regulatory RNAs that regulate gene expression post-transcription‐ ally. Among the altered miRNAs, miR940 was the most down-regulated one [13] Apart from their importance in myocardial tissue, miRNAs have been detected in blood of patients with various cardiovascular diseases, offering the possibility to use them as potential biomarkers [14,15,16,17,18]. In adult patients with dyspnea, circulating miR-423-5p has been identified as a potential biomarker that can distinguish patients with left-sided heart failure from those with other causes of dyspnea [19] This miRNA failed as a biomarker in patients after atrial switch operation for transposition of the great arteries [20]. We aim to identify the specific patterns of circulating miRNAs in blood of patients with repaired, non-syndromic TOF and to assess whether these specific miRNAs may be useful to differentiate patients with and without heart failure
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