Abstract
Serum miRNAs are potential biomarkers for predicting the progress of bone diseases, but little is known about miRNAs in alcohol-induced osteonecrosis of femoral head (AIONFH). This study evaluated disease-prevention value of specific serum miRNA expression profiles in AIONFH. MiRNA PCR Panel was taken to explore specific miRNAs in serum of AIONFH cases. The top differentially miRNAs were further validated by RT-qPCR assay in serum and bone tissues of two independent cohorts. Their biofunction and target genes were predicted by bioinformatics databases. Target genes related with angiogenesis and osteogenesis were quantified by RT-qPCR in necrotic bone tissue. Our findings demonstrated that multiple miRNAs were evaluated to be differentially expressed with high dignostic values. MiR-127-3p, miR-628-3p, and miR-1 were downregulated, whereas miR-885-5p, miR-483-3p, and miR-483-5p were upregulated in serum and bone samples from the AIONFH patients compared to those from the normal control individuals (p < 0.01). The predicted target genes of the indicated miRNAs quantified by qRT-PCR, including IGF2, PDGFA, RUNX2, PTEN, and VEGF, were presumed to be altered in necrotic bone tissue of AIONFH patients. The presence of five altered miRNAs in AIONFH patients may serve as non-invasive biomarkers and potential therapeutic targets for the early diagnosis of AIONFH.
Highlights
Serum miRNAs are potential biomarkers for predicting the progress of bone diseases, but little is known about miRNAs in alcohol-induced osteonecrosis of femoral head (AIONFH)
The quantitative RT-PCR (qRT-PCR) was performed to further validate the results, and our findings revealed that miR-127, miR-628-3p, miR-1, and miR-432-5p were downregulated (p < 0.05), miR-885-5p, miR-483-3p, and miR-483-5p were upregulated (p < 0.05)
MiR‐127‐3p was the most abundant miRNA in the samples of AIONFH patients with a 8.31‐fold change compared with the healthy control
Summary
Serum miRNAs are potential biomarkers for predicting the progress of bone diseases, but little is known about miRNAs in alcohol-induced osteonecrosis of femoral head (AIONFH). The top differentially miRNAs were further validated by RT-qPCR assay in serum and bone tissues of two independent cohorts. Their biofunction and target genes were predicted by bioinformatics databases. The presence of five altered miRNAs in AIONFH patients may serve as noninvasive biomarkers and potential therapeutic targets for the early diagnosis of AIONFH. MiRNAs are considered to be involved in the proliferation, differentiation, and apoptosis of osteoclasts, osteoblasts and osteocytes[13] They regulate the expression of mRNA in the posttranscription process by inducing the RNA silencing complex[14].
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