Analysis of Chromosomal Numbers, Mitochondrial Genome, and Full-Length Transcriptome of Onychostoma brevibarba

Fangzhou Hu,Yunfan Zhou,Qinbo Qin,Liangxiong Bai,Rurong Zhao,Ming Ma,Chun Zhang,Ming Zhu,Kaikun Luo,Chang Wu,Shi Wang,Shaojun Liu,Chenchen Tang,Aiguo Zhou,Min Tao,Bo Chen,Jingjing Fan,Jinpu Wang

doi:10.1007/s10126-019-09899-6

Abstract

Onychostoma brevibarba is a new discovered species which is distributed in Xiang Jiang River of the middle Chang Jiang basin in Hunan Province, South China. In this study, the ploidy levels of O. brevibarba were confirmed by counting chromosomal numbers and analyzing karyotype. The complete mitochondrial genome of O. brevibarba was determined and analyzed. Besides, we firstly performed the full-length transcriptome of O. brevibarba derived from 5 different tissues using the PacBio SMRT sequencing. The result shows that O. brevibarba was a diploid with 48 chromosomes. The complete mitogenome of O. brevibarba was 16,602 bp in size and very similar (89.1–91.3%) to that of other Onychostoma species but was distinct from all congeners. The full-length transcriptome dataset of O. brevibarba comprised 120,239 unigenes. Among the unigenes, 91,542 were functionally annotated, whereas 26,794 were found to have two or more isoforms. This study could provide many new insights into cytology and molecular characteristics of O. brevibarba; it laid the foundation for further exploration of the genomic signatures of species of Onychostoma.

Highlights

Species in the genus Onychostoma are small and mediumsized freshwater fishes that belong to the order Cypriniformes (Cyprinidae: Barbinae) that are found throughout hill streams and in middle-and upper-river habitats (Yue et al 2000)
Our result provides many new insights into cytology and molecular characteristics of O. brevibarba; it established the base of further research, utilization, and protection of this species
A total of 19 valid species of the genus Onychostoma had been recognized globally (Yue et al 2000; Yin and Dai 2014). Some of this species of Onychostoma were locally known to be of high economic value, even supporting a Library size m54136 0.5–6 K m54065 0.5–6 K

Summary

Introduction

Species in the genus Onychostoma are small and mediumsized freshwater fishes that belong to the order Cypriniformes (Cyprinidae: Barbinae) that are found throughout hill streams and in middle-and upper-river habitats (Yue et al 2000). Onychostoma contains approximately 19 species (subspecies) that are distributed widely in East and Southeast Asia, and some are of high economic value (Han et al 2015). The restriction of the lower lip, which bears a sharp, cornified sheath on the cutting edge, to the sides of the lower jaw in Onychostoma is almost diagnostic (Yue et al 2000). The research and evaluation of the genetic diversity of the species of Onychostoma have important practical guiding significance for the conservation and sustainable use of the fish resources

Objectives

Methods

Results

Conclusion