Abstract
A proteoglycan (PG) was separated from human urine by ion chromatography and gel filtration. With our sensitive determination method, the PG (M.W. Ca. 67, 000) was found to contain low-sulfated chondroitin 4-sulfate (Ch4S). The PG inhibited trypsin and had the same chromatographic behavior as human urinary trypsin inhibitor (HUTI), which also contained the low-sulfated Ch4S. From these results, it was concluded that the PG and HUTI were the same substance. The difference between individuals in the composition of the low-sulfated Ch4S in HUTI was also examined.
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