Abstract
: Chloramphenicol (CAP) is a widely used antibiotic for bacterial diseases for animals and in typhoid fever in human beings. SPE-HPLC method is described for the detection of chloramphenicol from human blood plasma samples. Frusemide (FRU) was taken as an internal standard to determine the percentage recovery of chloramphenicol in blood sample. Their standard solutions were prepared in methanol having the concentration of 1.0 mg/mL. The extraction of both the drugs was carried out by SPE using methanol at a flow rate of 0.1mL/min. Further, the analysis was done by HPLC having monolithic, chromolith performance RP-18e, 100-4.6 (100 × 4.6 mm) column. Phosphate buffer (100 mM, pH 2.5)-acetonitrile (75:25, v/v) was selected as a mobile phase at a flow rate of 1.5 mL/min. Detection of the drugs were carried out at 270 nm. The percentage recoveries of CAP and FRU from plasma samples were 92.0 and 90.0, respectively. The detection limit of the developed method for CAP and FRU were 0.8 µg/mL and 1.0 µg/mL, respectively. The result of separation factor (α) and resolution factor (Rs) of CAP with respect to FRU in plasma samples were 2.31 and 16.25, respectively. The developed method may be used for quantification and detection of CAP from samples of biological origin.
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