Abstract
Article history: Sintoc bark (Cinnamomum sintoc Bl) belongs to Lauraceae (the laurel family). It has been used empirically for a treatment for swelling caused by insects' bites. In this study, the research examined the analgesic activity and anti-inflammation of essential oil of sintocbark using in vivo methods. The mechanism of anti-inflammation was predicted using molecular docking against COX-2. Essential oil of sintoc bark was collected by distilling through steam distillation, and then analyzed by GC-MS. Analgesic and anti-inflammatory activity was examined by in vivo, which were conducted by writhing and carrageenan-induced methods, respectively. The findings showed that the tested sintoc bark oils contained 36 components of essential oil with eugenol (38.38 %) as a major compound. In the in vivo experiments, sintoc bark oils with doses 0.005 mL, 0.010 mL, and 0.020 mL/20g body weight significantly (p<0.05) reduced the number of writhing of mice when compared to negative control group. All of doses of sintoc bark oils gave significantly affect (confidence level 99 %) compare to negative control. Sintoc oil with dose 0.2 ml/200g had the strongest inhibition compare to positive control (indometasin 10 mg/kg body weight). The molecular docking results indicated that the compounds of aryl propanoid were generally potential to inhibit COX-2.
Highlights
Inflammation is a mechanism of the body’s defense caused by the tissue response to such detrimental effects-both local or inside the body (Nathan, 2002)
The findings showed that the tested sintoc bark oils contained 36 components of essential oil with eugenol (38.38 %) as a major compound
Tools To analyze the essential oil with Gas Chromatography-Mass Spectrometry method and examine the inhibitory activity of COX-2, the tools used are microplat, CO2incubator (Sanyo), Laminar Air Flow (LAF) (Jeio Tech), High Speed Refrigerated Centrifuge (Union 32 R), otoklaf (Memert), cells counter (Improved Double Nubauer depth 0,1 mm) microscope (Niken Eclipse 80) and microplate reader (Benchmark)
Summary
Inflammation is a mechanism of the body’s defense caused by the tissue response to such detrimental effects-both local or inside the body (Nathan, 2002). The effects can be devastating to physics, chemistry, bacteria, parasites and so forth. Physical damage can be caused by high temperature, light and radiation, including foreign objects inside the organ, or any causes that prompt devastating effects. Strong bases and toxins immerse in chemical causes. Pathogenic bacteria of Streptococcus, Staphylococcus and Pneumococcus genus, for instance, are devastating (Khansariet al., 2009). Inflammatory reaction can be observed from clinical symptoms around affected tissue, such as increase in heat (calor), reddish
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