Abstract
The authors studied the effects of cyclophosphamide and alpha-interferon on the natural cytostatic and cytotoxic activity of mouse spleen cells on lymphoma cells. The cells were incubated in medium RPMI-1640 with 10% fetal serum and 1% glutamine in the presence of tritiated thymidine. Incorporated radioactivity was estimated by scintillation counting. The natural cytotoxic activity of the cells was determined by the release of chromium 51 from labelled lymphoma cells transplanted in vitro. Pretreatment of spleen cells with leukocytic interferon was found to cause an increase in both cytotoxic and cytostatic activity of these cells. An investigation of the time course of the antimitotic activity of the cells after pretreatment with cyclophosphamide, causing the death of proliferating cells, showed that effectors of natural cytotoxic activity constitute a homogeneous group as regards sensitivity to cyclophosphamide. Cells exerting cytostatic activity in vitro are evidently heterogeneous for sensitivity to cyclophosphamide. Thymus and bone marrow cells were used for comparative purposes.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
