Abstract
Background Sensor proteins that detect cGMP are valuable tools to analyse the molecular mechanisms underlying cGMP’s manifold physiological functions. These cGMP sensors allow for visualization and quantification of cGMP in living cells, in real time, and at high spatial and temporal resolution. For instance, they allow one to study sub-cellular cGMP compartments, and combined with multi-photon microscopy, they can provide new insights into complex biological processes, which can be analysed only in live animals.
Highlights
Sensor proteins that detect cGMP are valuable tools to analyse the molecular mechanisms underlying cGMP’s manifold physiological functions
One mouse line produced by random transgenesis expresses cGi500 in vascular and visceral smooth muscle
Other mouse lines were created by targeted modification of the ROSA26 locus; these lines either show ubiquitous cGi500 expression, or they can be used for Cre/lox-dependent, tissue-specific cGi500 expression
Summary
Analysis of cGMP signalling with transgenic mice expressing FRET-based cGMP sensors. Lai Wen1†, Martin Thunemann1†, Susanne Feil, Matthias Hillenbrand, Angelos Vachaviolos, Thomas Ott, Xiaoxing Han, Dai Fukumura, Rakesh K Jain, Cor de Wit, Michael Russwurm, Robert Feil1*. From 6th International Conference on cGMP: Generators, Effectors and Therapeutic Implications Erfurt, Germany. From 6th International Conference on cGMP: Generators, Effectors and Therapeutic Implications Erfurt, Germany. 28-30 June 2013
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