Abstract
SUMMARY The main constituents of the cell wall of Saccharomyces cerevisiae, i.e. β-1,3-glucan, β-1,6-glucan and mannan, could be distinguished by pyrolysis ammonia chemical ionization mass spectrometry or Py(CI)MS. Cell walls extracted with alkali, which removes the mannan fraction and part of the glucan from the cell wall, yielded mass spectra that differed clearly from nonextracted cell walls. Multivariate analysis of these spectra showed a shift from ‘mannan-like’ towards ‘glucan-like’ cell walls. Known cell wall mutants, such as mnn9 and kre1, were recognized by this technique. Py(CI)MS in combination with multivariate analysis of the spectra is therefore a useful tool for identifying and characterizing cell wall mutants in yeast.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
