Analysis of Carbohydrates in Newly Developed Liquid State Rabies Vaccine

Jagannathan S,Vijayakumar R

doi:10.4172/1948-5948.1000040

Abstract

Rabies is an under- reported, neglected deadly disease estimated to cause more than 50,000 human deaths annually, most of which occur in the poorest regions of the world. One of the most elements in the effective control of human rabies is the use of ef fi cacious vaccines. The stabilizers play an important role in the ef fi cacy of the vaccines. Our present study emphasizes on the analysis of stabilizers, trehalose and lactose by various biochemical methods in the newly developed VERO cell line based Tissue culture anti liquid rabies vaccine.

Highlights

Vaccines are widely used as highly cost-effective tools for improving health and have for the last decades had a major impact on public health (Bloom et al, 2005)
Since Louis Pasteur’s discovery of the rabies vaccine, rabies has been a disease that can be prevented through the timely administration of post-exposure prophylaxis (PEP)
Investigators have been searching for alternative rabies vaccines having all the advantages of the killed or modified live rabies virus vaccines for total stimulation of the immune response in animals and humans, without any undesirable side effects sometimes associated with conventional rabies virus vaccine

Summary

Introduction

Vaccines are widely used as highly cost-effective tools for improving health and have for the last decades had a major impact on public health (Bloom et al, 2005). The types of material that are added to vaccines as stabilizers include sugars such as sucrose, trehalose, maltose, fructose, glucose, sorbitol and lactose amino acids , proteins such as human serum Albumin (HSA) or gelatin (WHO 2006). Stabilizers are materials that help to protect the vaccine from adverse conditions such as freeze-drying process Carbohydrate such as sucrose, lactose, glucose, trehalose and maltose are used as cryoprotectants and as a stabilizer and preservative. Micro needles prepared without trehalose elicited a strong antibody response after just one day of strong but showed lower levels of influenza virus specific Ig-G after 1 week or 1 month storage time It showed that influenza vaccine loses immunogenicity during storage and that the presence of trehalose can prevent that loss. Our study is dealing of the determination of the carbohydrates present in the newly developed liquid rabies vaccine by the utilizing the traditional as well as newer assay techniques

Materials and Methods

TCALRV-D

Results and Discussion