Analysis of calorimetric data for the binding of monomeric bis-benzimidazole, an analog of the Hoechst 33258 dye, to poly(dA) · poly(dT)

Abstract

Monomeric bis-benzimidazole (MB) is an analog of the Hoechst 33258 dye. The enthalpy and entropy of MB binding were evaluated by analyzing the calorimetric data on MB reverse titration with poly(dA) · poly(dT). A mathematical model was developed to estimate the thermodynamic parameters of binding on the basis of calorimetric data. The results agree well with spectrophotometric data on the binding of analogous compounds. The model was used to estimate the parameters of binding with poly(dA) · poly(dT) for dimeric bis-benzimidazole (DB), which consists of two bis-benzimidazole monomers linked via a flexible chain. The ligand was assumed to produce different types of complexes with the polymer.