Abstract
The objective of the present work is to design and implement a low cost PIC18F452 microcontroller based instrument for the measurement of calcium in milk samples using Light Emitting Diode (LED) as source and photodiode as detector. The developed instrument measures the absorbance, calculates the concentration and displays the results in Liquid Crystal Display (LCD). The principle of the measurement is based on the reaction between calcium and Ortho Cresolphthalein Complexone (OCPC) reagent in alkaline medium to form purple complex with maximum absorption at 570 nm. Algorithm is developed to monitor and control the process sequences and transmit data to PC via serial communication module using an RS232 protocol. Statistical analyses are carried out to evaluate the performance characteristics of the developed instrument and compared with the conventional instrument. Linear calibration curve is obtained between 0 and 5.0 mmol L−1 and lower limit of detection is 0.05 mmol L−1. The developed system shows the good performance and the results are in good agreement with the current clinical spectrophotometric method at 96% of confidence level.
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