Analysis of biological pretreatment of rapeseed straw with white rot fungi for enzymatic hydrolysis

Rasool Ghasemzadeh,Mohammad Taghi Hamed Mosavian,Afzal Karimi

doi:10.4067/s0718-221x2018005041801

Abstract

The effect of biological pretreatment on the rapeseed straw was evaluated by solid-state cultivation of white rot fungi Phanerochaete chrysosporium. P.chrysosporium degraded the lignin during the pretreatment, with enzymatic hydrolysis ratios increasing in the pretreated straw (3-fold after a 15 day pretreatment). The samples were identified by XRD, FTIR and SEM. X-ray analysis showed that pretreated samples had higher crystallinity than untreated samples (39.47% for a pretreated sample compared to 33.17% for untreated samples) and FTIR spectroscopy demonstrated that the content of lignocellulose also decreased during the biological pretreatment process. The surface characterization study showed morphological changes in pretreated samples. Moreover, the biological pretreatment slowed a plunge in hydrolysis rate during enzymatic hydrolysis.

Highlights

The third widely used oil in the world is rapeseed (Brassica napus) oil
The effect of biological pretreatment on rapeseed straw chemical composition Lignin is an important compound found in plant cell walls that limits chemical and biological decomposition and hydrolysis
The results are in line with previous studies in which biological pretreatment of lignocellulosic materials had been investigated by white rot fungi (Xu et al 2010, Singh et al 2011, Deswal et al 2014)

Summary

Introduction

The third widely used oil in the world is rapeseed (Brassica napus) oil. There has been a significant increase in the application of rapeseed oil to produce biodiesel. According to FAO (2016), rapeseed was cultivated in over 36 million hectares farms around the world in 2014 with Iran’s share being about 160000 hectares. After harvesting and separating rapeseed seeds, agricultural residues are usually burned. Due to its lignocellulosic nature, the rapeseed straw can be utilized for fuel ethanol production via a biochemical process. This process includes pretreatment, enzymatic hydrolysis, and fermentation. Enzymatic hydrolysis is an important step in changing lignocellulosic materials into biofuel (Dionisi et al 2015)

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Results

Conclusion