Abstract
In this study, a capillary zone electrophoretic method, in which 1-alkyl-3-methylimidazolium-based ionic liquids (IL) was used as the additive, was established to separate and identify six bioactive flavonoid-O-glycosides in Saussurea mongolica Franch. In order to investigate the traits of IL in capillary electrophoresis (CE), the influences of the alkyl group, the anionic part and the concentration of IL were investigated and discussed. Optimum separation was achieved with 20 mM borate buffer at pH 9.00 containing 5 mg/mL 1-ethal-3-methylimidazolium tetrafluoroborate. The applied voltage was 15 kV and the capillary temperature was kept constant at 25°C. The relative standard deviations (RSD) of the migration time and the peak area of each peak for six replicate injections were 0.75%-1.45% and 3.18%-3.91%, respectively. Moreover, it was worth noticing that the mechanism of interaction between IL and analytes was investigated preliminarily.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
