Abstract
Coconut shell (CS) is one waste that can be processed to produce bio-oil by pyrolysis method to be used as an active ingredient of biopesticides. The purpose of this study is to analyze the effectiveness of bio-oil from coconut shells as biopesticides by optimizing the performance of potentiometric biosensors based on acetylcholinesterase enzymes including: % inhibition test, range of work concentration, Limit of detection (LoD) and accuracy. The results of the measurement of percent inhibition of the concentration of natural pesticides from the lowest concentration of 10-6 to 10-2 % (v/v) obtained the percent inhibition value respectively were 62.16; 58.08; 54.75; 49.15; 38.99; 26.98 and 17.27 %. The results of the analysis of the concentration range of work showed a linearity with a correlation coefficient of 0.951 in the measurement range of 10−8% 10−2 % (v/v). LoD in the range of 107 % (v/v) and accuracy obtained an average value of percent recovery of 98.17 %, therefore testing the effectiveness of biopesticides can be done using potentiometric biosensors based on the acetylcholinesterase enzyme.
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