Analysis of bile acids in serum and bile by capillary gas-liquid chromatography.

G Karlaganis,G Paumgartner

doi:10.1016/s0022-2275(20)41279-9

Abstract

Various liquid phases for glass capillary columns have been evaluated for gas-liquid chromatographic analysis of methyl ester trimethylsilylether derivatives of bile acids from serum and bile. Bile acid analysis is rapid and exhibits high separation efficiency with a 20 X 0.3 mm glass capillary column whose internal surface is covered with a crystal layer of barium carbonate and coated with polyethyleneglycol 20000 as liquid phase according to Grob et al.

Highlights

Supplementarykey words glass capillary column cholesterol trimethylsilylether derivatives Gas-liquid chromatography of bile acid derivatives on packed columns is a well established and widely used method (1)
OV-101 and OV-61 glass capillary columns were manufactured by H. and G
Methyl ester TMS derivatives of bile acid standards were injected into the same polyethyleneglycol 20000 glass capillary column at different days over a period of 3 months

Summary

Georg Karlaganis and Gustav Paumgartner

Summary Various liquid phases for glass capillary columns have been evaluated for gas-liquid chromatographic analysis of methyl ester trimethylsilylether derivatives of bile acids from serum and bile. Bile acid analysis is rapid and exhibits high separation efficiency with a 20 X 0.3 mm glass capillary column whose internal surface is covered with a crystal layer of barium carbonate and coated with polyethyleneglycol20000 as liquid phase according to Grob et al (Chromatographia 10: 181, 1977). Supplementarykey words glass capillary column cholesterol trimethylsilylether derivatives Gas-liquid chromatography of bile acid derivatives on packed columns is a well established and widely used method (1). Lithocholic acid and cholesterol could not be separated on this column (3). We have tested glass capillary columns with different polar stationary phases for analysis of bile acids in serum and bile.

Materials and methods

Absolute Retention Relative Retention

Results and discussion

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