Abstract
In parallel with the appearance of new dental materials the number of induced allergic diseases increases. Based on this fact more sensitive detection of allergens is major importance. The Fourier-Transform Surface Plasmon Resonance (FT-SPR) is a sensitive, broadly applicable real-time method for analysing thin layers of materials on gold surfaces. FT-SPR measurement is performed at a fixed angel of incident light, and reflectivity is measured over a range of wavelength in the near infrared. In our study the formaldehyde and benzoyl-peroxide were examined as members of the most common dental allergens by FT-SPR spectroscopy. The aim of this work was the investigation of the suitability of this method for the direct detection of these materials. Different concentrations of formaldehyde and benzoyl-peroxide solutions were measured from this purpose. The individual spectra were measured for all of the solutions, and calibration curves were calculated for the materials for the possibility of the determination of an unknown concentration. In addition, series measurements were performed whereby the association and dissociation properties of formaldehyde or benzoyl-peroxide were described. The results of the experiments proved that the method capable to measure directly these materials and can provide appropriate calibration curves for determination of unknown concentrations.
Highlights
Surface plasmon resonance (SPR) is a surface sensitive optical technique that is sensitive to the thickness and refractive index of thin layers at the interface between a metal surface and bulk medium
The SPR angle varies with the refractive index of the material close to the surface on the side opposite from the reflected light
Fourier-Transform Surface Plasmon Resonance (FT-SPR) measurement is performed at a fixed angel of incident light, and reflectivity is measured over a range of wavelength in the near infrared
Summary
Surface plasmon resonance (SPR) is a surface sensitive optical technique that is sensitive to the thickness and refractive index of thin layers at the interface between a metal surface and bulk medium. This instrument method focuses on the determination of biological activity rather than biological structure [1, 2]. SPR is conventionally performed by measuring reflectivity over a series of angles of incident light of fixed wavelength. The SPR angle varies with the refractive index of the material close to the surface on the side opposite from the reflected light.
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