Abstract
We report the new application of negative ion atmospheric pressure chemical ionization (APCI) to the detection of composite bacteriohopanepolyols in bacterial and sedimentary (or other natural environmental) samples. The method employs the addition of dichloromethane to the high performance liquid chromatography eluent prior to APCI to stimulate formation of chlorine addition/association ions from the hopanoid alcohols. This approach provided the first direct analysis of composite bacterial hopanoids in a sediment, identifying bacteriohopanetrol and bacteriohopanetetrol cyclitol ether. © 1998 John Wiley & Sons, Ltd.
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