Abstract

The redox balance in a variety of Gram-negative bacteria was explored using redox sensitive GFP (roGFP2), J. van der Heijden et al. doi:10.1016/j.freeradbiomed.2015.11.029[1]. This data article provides Supporting material to further investigate the relationship between Salmonella typhimurium survival and oxidative stress. The first set of data presented in this article, shows the percentage of surviving bacteria after exposure to hydrogen peroxide. The second set of data shows the concentration of hydrogen peroxide that was produced by S. Typhimurium in different growth phases. The last set of data shows the percentage of surviving S. Typhimurium bacteria after exposure to different antibiotics.

Highlights

  • The survival of Salmonella typhimurium after exposure to hydrogen peroxide was analyzed under a variety of different conditions by determining colony forming units

  • Data After growing Salmonella Typhimurium bacteria under a variety of environmental conditions, bacterial survival was determined after exposure to oxidative stress

  • The production of endogenous hydrogen peroxide was measured of bacteria that were grown to different growth phases

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Summary

Data accessibility

Graphs Fluorescent plate reader (Tecan Trading AG, model: Infinite M200) to measure fluorescence for the Amplex red assay and colony forming units to assess bacterial survival Analyzed Salmonella typhimurium (expressing roGFP2) was grown under various conditions to late exponential phase or stationary phase prior to the experiments. The survival of Salmonella typhimurium after exposure to hydrogen peroxide was analyzed under a variety of different conditions by determining colony forming units. The percentage survival of Salmonella typhimurium was determined after exposure to several antibiotics by colony forming units. The data identifies environmental conditions that affect bacterial survival after exposure to oxidative stress. After growing Salmonella Typhimurium bacteria under a variety of environmental conditions, bacterial survival was determined after exposure to oxidative stress. The production of endogenous hydrogen peroxide was measured of bacteria that were grown to different growth phases.

Bacterial strain
Survival experiments after growth in different growth media
Survival experiments under different pH
Survival experiments in different temperatures
Findings
Survival experiments after exposure to different antibiotics
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