Abstract
Тhe study of single-nucleotide polymorphisms of Toll-like receptors has an important applied and theoretical value for revealing the mechanisms in formation of immunity features and its correction. The aim of the research was to study the available frequency of polymorphic variants of the TLR2 gene (rs5743708) and 896A/G (rs4986790), 1196C/T (rs4986791) of the TLR4 gene, and to assess the association with the health status of apparently healthy individuals. Materials and methods: the study involved 114 Caucasian individuals living in Poltava or Poltava oblast for a minimum of 2 years, who underwent the collection of anamnestic data, as well as the data of objective and clinical examinations. The polymorphic sites of the TLR2 (rs5743708) and TLR4 genes (rs4986790, rs4986791) were determined by polymerase chain reaction (PCR) followed by analysis of the restriction fragments length of the PCR products. The results of distribution of polymorphic variants 2258G/A in the genotypes of the TLR2 gene (rs5743708), 896A/G of the TLR4 gene (rs4986790), and 1196C/T of the TLR4 gene (rs4986791) corresponded to the theoretically expected ones at the Hardy-Weinberg equilibrium (χ2 = 0.02, р = 0.99; χ2 = 0.29, р = 0.86; χ2 = 1.46, р = 0.48, respectively). When comparing the presence of individual clinical manifestations that were detected during the interviewing, with the presence of polymorphic alleles in the genotype, a reliable relationship was established between the presence of the A allele in the polymorphic version of the TLR2 gene (rs5743708) in the genotype with rheumatism (p = 0.05), pyelonephritis (p = 0.05) and a bad habit of smoking (p = 0.04).
Highlights
Тhe study of single-nucleotide polymorphisms of Toll-like receptors has an important applied and theoretical value for revealing the mechanisms in formation of immunity features and its correction
A large number of research publications covering the problem of recognizing foreign pathogens prove the leading role of Toll-like receptors (TLRs) in this issue, but sometimes contain controversial data regarding the influence of polymorphic variants of genes encoding these receptors on the recognition of the corresponding ligands and the intensity of the immune response [2]
Genetic variability of TLRs can determine the differences in the susceptibility of organisms to bacteria and viruses, the intensity of the inflammatory process [3]
Summary
Тhe study of single-nucleotide polymorphisms of Toll-like receptors has an important applied and theoretical value for revealing the mechanisms in formation of immunity features and its correction. Toll-like receptors (TLRs) belong to the class of transmembrane single-chain signal receptor proteins that recognize foreign evolutionarily conservative molecular structures of pathogens, known as pathogen-associated molecular structures and trigger the cascade of sequential reactions of congenital and antigen-specific adaptive immunity through signaling pathways (MyD88-dependent and TRIF-dependent), resulting in the expression of genes of proinflammatory cytokines and modulation of the inflammatory response of the body [1]. A large number of research publications covering the problem of recognizing foreign pathogens prove the leading role of TLRs in this issue, but sometimes contain controversial data regarding the influence of polymorphic variants of genes encoding these receptors on the recognition of the corresponding ligands and the intensity of the immune response [2]. The association between the presence of polymorphic alleles in 2258A genotype of the TLR2 gene and 896G of the TLR4 gene with the development of allergic rhinitis has not been established [8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
