Abstract
A reversed-phase high-performance liquid chromatography (HPLC) system has been developed and optimised for the separation of aryltetrahydronaphthalene lignans of the podophyllotoxin series and their glucoside conjugates. The separations were carried out on an ODS-Hypersil column with CH 3OH-water, CH 3CN-water, CH 3OH-ammonium acetate or CH 3CN-ammonium acetate as the mobile phase. The optimised HPLC system has been applied to the analysis of lignans in podophyllin resins. The separations showed the presence of podophyllotoxin, 4′-demethylpodophyllotoxin, podophyllotoxin δ- d-glucoside and 4′-demethylpodophyllotoxin-β- d-glucoside in Podophyllum emodi resin, while in Podophyllum peltatum resin, α- and β-peltatin and their glucoside conjugates were also detected, in addition to podophyllotoxin.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
