Abstract
The bioavailable arsenic (As) content of contaminated soils was determined by joint analyses of acid-soluble, total water-soluble, and biovailable As by using a luminescent bacterial sensor, Escherichia coli MC1061(pTOO31). According to the results of this study, a significant positive correlation was found between the concentration of total water-soluble As and the bioavailability of As. However, the bioavailability of As in soil varied between sampling sites and was not equal when compared to the concentration of total water-soluble As; bioavailable As was 3 to 77% of total water-soluble As in soil. Our experiments also showed that aging and sequestration of As occurs in contaminated soils and As compounds thus become progressively less bioavailable with time. As a consequence, the bioavailability and toxicity of As should be considered when evaluating the ecological risks of contaminated soils.
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