Abstract
The present study examined the changes of karyomorphism in cultured cells (human colon adenocarcinoma cell line HT-29 and human oral squamous cell carcinoma cell line KB) using a confocal laser scanning microscope (ACAS 570 laser cytometer). After INF-γ stimulation, Apoptosis of HT-29 cells and KB cells were induced by the Fas/Fas ligand system and cychroheximide, respectively. Both types of apoptosis-induced cells were stained with propidium iodide (PI) and distribution of nuclear DNA content was determined, and the three-dimensional image was analyzed. The results were as follows. Simultaneous visualization of quantitative and morphological changes of DNA, using PI fluorescence as an indicator, clarified that the distribution of DNA content in the nucleus was remarkably uneven; it was low in the central area and high in the marginal area. In addition, three-dimensional images revealed a concave area in the center of the cell nuciei, fragmented irregular nuclei, and a doughnut-shaped and hemispheric form. Thus, the three-dimensional study presented a clear picture of the nuclear structures which characteristically appear in cells during the process of apoptosis. Confocal laser scanning microscopy was confimed as useful in the observation of apoptosis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
