Abstract
To investigate the therapeutic potentials of na- tural sources, stepwise polarity fractions of Blumea balsamifera were tested for their ability to inhibit aldose reductase (AR) activity in rat lenses. Of these, the ethyl acetate (EtOAc) fraction exhibited a unique AR inhibitory activity (IC50 value, 0.11 μg/mL). Apigenin was identified from the active EtOAc fraction and exhibited high AR inhibitory activity (IC50 value, 4.03 μM). The content of apigenin was measured in B. balsamifera (0.47 mg/g) by HPLC/UV analysis. Our result suggests that B. balsamifera could be a useful natural source for the development of a novel AR inhibitory agent against diabetic complications.
Highlights
Blumea balsamifera belonging to Asteraceae family is a flowering plant that is widely distributed in open fields, grasslands, and waste areas at low and medium altitudes in regions of Southeast Asia, such as Southern China and the Philippines [1]
Validation of the HPLC method for compound 1 as a standard compound was determined by Limit of Detection (LOD) and Limit of Quantification (LOQ)
The EtOAc fraction showed the strongest inhibitory activity with an IC50 value of 0.11 μg/mL, which is comparable to the commercial synthetic aldose reductase (AR) inhibitor tetramethyleneglutaric acid (TMG) (IC50 = 0.84 μg/mL)
Summary
Blumea balsamifera belonging to Asteraceae family is a flowering plant that is widely distributed in open fields, grasslands, and waste areas at low and medium altitudes in regions of Southeast Asia, such as Southern China and the Philippines [1]. Smells are strongly of camphor when bruised [2] This plant has numerous uses in traditional Thai and Chinese medicine, including the treatment of septic wounds and kidney disorders [2,3,4,5]. B. balsamifera has attracted attention as a medicinal plant as it possesses various physiological activities such as anti-obesity [9], plasmininhibitory [1], anti-fungal [8], liver-protective [10], and anti-cancer effects [11]. Up to our knowledge, it has not been reported to evaluate the aldose reductase (AR) inhibitory activity of natural compounds in B. balsamifera. As a preliminary step for the evaluation of the potential of naturally occurring AR inhibitors from B. balsamifera, we tested the effects on rat lens AR inhibition and the quantities of the compound
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