Analysis of antioxidant status (Superoxide dismutase and Catalase) in primary and secondary infection of Eimeria tenella in chicken

Abstract

In the present study, Superoxide Dismutase (SOD) concentration and Catalase (CAT) activity in caecal tissue of broiler chicken was determined in primary and secondary infection of Eimeria tenella. Primary infection groups were infected once with 1×104sporulated E. tenella oocysts at 3rd week of age. Secondary infection with 1×104 sporulated oocysts was performed at day 14 post- primary infection in the secondary infection group. Chickens were sacrificed by cervical dislocation at 0 hour, 2, 6, 12, 24, 48, 72 and 96 hour post primary and secondary infection and the caeca were removed. SOD and CAT enzyme were estimated from the caecal tissue homogenate. In this study, it was observed that SOD and CAT, both the enzymes were increased in initial stage of primary and secondary infection, where as in later stage of primary infection SOD was increased and CAT activity reduced, and in secondary infection vice versa.