Analysis of Antioxidant Capacity of Chromones in Saposhnikoviae Radix Obtained by Ultrasonic-Assisted Deep Eutectic Solvents Extraction.

Xianwen Yue,Fangfei Xu,Huiwei Bao,Peng Lv,Huailei Yang,Yang Xu,Pablo Richter

doi:10.1155/2020/8875788

Abstract

In this paper, ultrasonic-assisted deep eutectic solvent (DES) extraction was applied to the acquisition of chromones (cimicifugin, prim-o-glucosylcimifugin, and 5-o-methylvisamminoside) from Saposhnikoviae radix (SR). The extraction effects of 11 prepared DESs were screened taking contents of chromones as indexes. Furthermore, the optimum extraction conditions were confirmed using a single-factor test and response surface optimization test. Scavenging activities of DPPH anion and ABTS cation radicals of different SR extracts (DES, methanol, and ethanol) were studied. The analysis results of best extraction conditions optimized by Design-Expert software were as follows: extraction time (40 min), extraction temperature (60°C), and the solid/liquid ratio (32 mL/g). Scavenging rates of the DES extract for DPPH anion radical and ABTS cation radical were found to be 75.31% and 65.71%, which were higher than those of methanol and ethanol extracts. In conclusion, the developed extraction method can be regarded as a safe, green, and more effective approach for the extraction of chromones in SR.

Highlights

Saposhnikoviae radix (SR) is derived from the dry roots of Saposhnikovia divaricata (Turcz.) Schischk without flower and stem. e flavor of SR is pungent and slightly sweet, and the nature is warm
SR is a traditional Chinese medicine with a long history, which was listed as the top grade in the “Herbal Classic of Shennong.”
Some deep eutectic solvent (DES) cannot be regarded as extraction solvents due to their solid or viscous liquid status

Summary

Introduction

Saposhnikoviae radix (SR) is derived from the dry roots of Saposhnikovia divaricata (Turcz.) Schischk without flower and stem. e flavor of SR is pungent and slightly sweet, and the nature is warm. SR has the effects of inducing diaphoresis to dispel wind, relieving convulsion, and overcoming dampness [1]. SR is mainly used for the treatment of exogenous wind-cold, aches all over the body, headache, dizziness, and damp retention [2]. Prim-o-glucosylcimifugin (POG) and 5-o-methylvisamminoside (5OM) can decrease the temperature of febrile rats and has obvious antipyretic, analgesic, anti-inflammatory, and antiplatelet aggregation effects [7]. The content of cimicifugin (CIM) is found to be the highest in plasma. Since glycosides in SR (including POG and 5OM) must be converted into CIM in the intestine before being absorbed [8]. Erefore, CIM, POG, and 5OM, the three most representative chromones, were selected as the index components for the assessment of extraction effects of SR Since glycosides in SR (including POG and 5OM) must be converted into CIM in the intestine before being absorbed [8]. erefore, CIM, POG, and 5OM, the three most representative chromones, were selected as the index components for the assessment of extraction effects of SR

Methods

Results

Conclusion