Analysis of antibody-independent binding of dengue viruses and dengue virus envelope protein to human myelomonocytic cells and B lymphocytes

Abstract

The identification of cell surface receptor molecules for the dengue viruses, one of the leading causes of morbidity and mortality in tropical and subtropical parts of the world, remains controversial. Both glycoproteins and glycosaminoglycans have been identified as likely candidates on various cell types. However, most of these studies have used cell types other than those thought to be the main target cells in humans: monocyte-macrophages, B lymphocytes and bone marrow cells. In this report characterization of dengue virus binding to two human leukocyte cell lines, the myelo-monocytic cell line HL60 and a non-EBV transformed B cell line, BM13674, is described. The results corroborate earlier descriptions of the presence of virus-binding protein(s), different from the FcR, on the surface of human leukocytes, and further suggest that the proteins may have differential affinity for the four dengue virus serotypes in the order dengue 2≥dengue 3>dengue 1>dengue 4 virus.