Abstract
Capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MECC) have been employed for the separation of ampicillin and its degradation products, especially the oligomers of ampicillin which are readily formed during storage and in aqueous solution. Both CZE and MECC are suitable for the separation of the oligomers of ampicillin. MECC is, however, more suitable for the separation of other degradation products. Electrophoresis conditions used for the analysis of ampicillin and its degradation products were as follows: length of fused (uncoated) silica capillary 44 cm (36 cm effective length), 50 μm i.d.; voltage, 15 kV; temperature, 25°C; detection wavelength, 215 nm; separation buffer, 40 mM sodium dihydrogen phosphate, and 100 mM sodium dodecyl sulfate (SDS) adjusted to pH 7.5. The influence of various parameters on the separation such as SDS concentration and the pH of the buffer were investigated. The method shows good repeatability, linearity and sensitivity. The robustness of this method was examined by applying a full factorial design to test the influence of the pH of the buffer, the SDS concentration and the buffer concentration.
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