Abstract
ABSTRACTAmino acid analysis of three legume samples using precolumn phenylisothiocyanate (PITC) derivatization and reversed‐phase high performance liquid chromatography (RP‐HPLC) was compared with literature data or that obtained by the conventional ion‐exchange chromatographic (IEC) method. The results indicated that the amino acid profiles obtained by PITC derivatization and RP‐HPLC compared fairly well with literature data for soy isolates, and fairly with that obtained by the IEC method for navy beans. Average coefficient of variability of all amino acids determined by the PITC method was 6%. It was not essential to remove lipids (2.1%) in navy beans prior to acid hydrolysis and PITC derivatization for the analysis of amino acids.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
