Abstract
CE-C4 D methods for the analysis of amino acids (AAs) are presented. Combining the results from two methods with acetic acid and cyclodextrin-based BGEs, 20 proteinogenic AAs could be analyzed using CE. CE-C4 D was also, for the first time, applied to analyze free AAs in samples of mammalian cell culture supernatant. After dilution as only sample preparation, combining the results of the two CE methods allowed monitoring the concentration changes of 17 AAs in samples taken during the cultivation of CHO cells.
Highlights
Division of Applied Physical Chemistry, Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden
CE-LIF has been applied to monitor the uptake of 14 amino acids (AAs) during beer fermentation [3], and in another contribution an online monitoring system including microfluidic components for cell counting and filtration followed by CE separation of the metabolic markers glucose, Gln, Leu/Ile and lactate was developed [4]
Samples taken 1 h after the reactor was inoculated with the cells, and after 4 and 8 days of the process, respectively, were analyzed using CE-derivatization can be avoided (C4D)
Summary
CE-C4D was for the first time, applied to analyze free AAs in samples of mammalian cell culture supernatant. After dilution as only sample preparation, combining the results of the two CE methods allowed monitoring the concentration changes of 17 AAs in samples taken during the cultivation of CHO cells.
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