Abstract
Size-exclusion and ion-exchange high-performance liquid chromatography were used to monitor the presence of polymeric aggregates and of families of allergens of similar isoelectric point in Parietaria judaica pollen extracts. A radio-allergo-sorbent test and chromatofocusing were used for the analysis of individual fractions. This allows the detection of two groups of allergens, having p I of 4.7 and >5.7, respectively, and shows the effect of dialysis or ultrafiltration on the distribution of allergens.
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