Abstract
BackgroundAllergies caused by pollen from Populus deltoides are common, but the allergic components are still unclear.MethodsThe total proteins in pollen of P. deltoides were analyzed by proteomics, and the potential allergens were identified via the WHO/IUIS database and the allergenOnline database retrieval. One target protein was screened by bioinformatics and expressed in Escherichia coli. The biological activity of the expressed product was verified by animal experiments.ResultsThe total of 3929 proteins in pollen of P. deltoides were identified, and 46 potential allergens belonging to 10 protein families were recognized by database retrieval. B9N9W6 protein of Hsp70 family was screened by bioinformatics analysis and expressed successfully. ELISA showed that B9N9W6 can stimulate the immune system to produce specific IgE and promote the generation of IL-4. Flow cytometry showed that B9N9W6 can significantly stimulate the proliferation of CD4+ T cells and promote the polarization of Th2 cells. The pathological sections of mice lung tissues indicated that alveolar destruction was more severe in the B9N9W6 group than that of extract group, and there were more inflammatory cells infiltration, mucus exudation and bleeding.ConclusionB9N9W6 is an important antigenic substance in the pollen of P. deltoides. Due to the conserved structure of Hsp70 family, more attention should be paid to the possibility of sensitization when Hsp70 from any pathogenic species is administered.
Highlights
Allergies caused by pollen from Populus deltoides are common, but the allergic components are still unclear
The Gene Ontology (GO) annotation found that P. deltoides and P. tomentosa [14] have great similarities in biological process, cellular component and molecular function, but great differences in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway (Table 1)
Allergen specific IgE antibody is a major cause of type I allergic diseases, such as asthma [44], In the experiment, we detected specific IgE produced by B9N9W6 via Western blot, and the results suggested that B9N9W6 could produce specific IgE and had the ability to bind it (Fig. S1)
Summary
Allergies caused by pollen from Populus deltoides are common, but the allergic components are still unclear. The incidence of allergic asthma has been increasing in recent years [2]. At least 300 million people suffer from allergic asthma worldwide [3], which is highest among children [4, 5]. Pollen from plants is an important source of air-borne allergens, which seriously affects the quality of life for people who is susceptible to allergies [9]. In May of each year, mature pollen of P. deltoides is densely suspended in the air, which causes a mass of pollen to contact people’s eyes, nostrils, mouth and skin frequently, leading to tears, sneezing, itching and other symptoms. Allergic components in P. deltoides pollen remain largely unclear
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