Analysis of allelic variants in genes associated with meat productivity in regionalized small cattle breeds

Abstract

The purpose of the research was to study the polymorphism of allelic variants of genes associated with high meat productivity for further selection of parental pairs using genetic methods. Genotyping of the Dagestan mountain sheep breeds (broodstock and rams) was carried out using a polymerase chain reaction. Genotyping was carried out for the following genes: CAST, GH, GDF9 on a Tertsik thermal cycler. Analysis of the results of PCR carried out with samples of sires of the Dagestan rock breed indicates that the GH gene polymorphism is represented by the A allele, with a high (0.87) and allele B, with a low (0.13) frequency of occurrence. The GDF9 gene polymorphism is represented by the GDF9G allele with a high (0.82) and the GDF9A allele with a low (0.18) frequency of occurrence. The distribution of homozygous genotype GHAA and heterozygous GHAB reached 84% and 16%, respectively. At the same time, the homozygous GHBB* genotype was absent in the studied sample. As a result of the research, new knowledge was obtained on biodiversity and forms of associations of polymorphism of the genes calpastatin, somatotropin, differential growth factor; breed-specific, population-specific features of the allelic spectrum of the CAST, GH, GDF9 genes in sheep of the Dagestan mountain sheep breed were established.