Analysis of Alkaloid Components and Essential Oils in the Jasmine Tea and Insecticidal Effects of Ethanolic Extracts of Jasmine Tea on House Dust Mite

Abstract

GC/MS ingredient analysis and TD GC/MS analysis was performed to identify the components causing insecticidal activity among the variable components contained in jasmine tea. In the results, it was identified that the caffeine of 86.73% contained in the jasmine tea and the major essential oils in jasmine tea were delta-3-carene(23.61%), dllimonene(16.35%), 2-beta-pinene(14.70%) and alpha-pinene, (-)-(12.17%). It was examined the insecticidal effect of the ethanolic extracts of jasmine tea on house dust mites by concentrations(1.0, 0.5, 0.25, 0.125, 0.0625 ㎎/40㎕). At the concentration of extract 1 ㎎/40 ㎕ and 0.5 ㎎/40 ㎕, a mortality rate of 100% was observed. At the concentration of extract 0.25 ㎎/40㎕, the mortality rate of 91.25% was observed. At the concentration of extract 0.125 ㎎/40㎕, the mortality rate of 40% was observed. These results suggest that jasmine tea ethanol extract shows a high insecticidal effect (>91.25%) at concentrations of 0.25 ㎎/40 ㎕ or higher. The insecticidal effects of the caffeine standard and 9 kinds of essential oil standards contained in the jasmine tea were measured in order to verify the insecticidal effect of jasmine tea. The caffeine showed a high insecticidal effect (>74.09%) at concentrations of 0.125 ㎎/40 ㎕ or higher. The delta-3-carene showed a high insecticidal effect (>94%) at concentrations of 0.125 ㎎/40 ㎕ or higher and a 70.84% insecticidal effect at a concentration of 0.0625㎎/40㎕. 2-beta-pinene, trans-caryophyllene, and gamma-terpinene showed high insecticidal effects (>80%) at concentrations of 0.25㎎/40㎕ or higher.