Abstract
Bluegill sunfish ( Lepomis macrochirus) were exposed to a single dose of benzo[ a]pyrene by i.p. injection and maintained at 13 or 20°C. Acid hydrolysis of DNA isolated from the liver and hemoglobin from the erythrocyte, 72-h posttreatment, released tetrols, which were separated and quantitated by high-performance liquid chromatography/fluorescence analysis. Examination of the tetrol profiles indicated that adduct formation with both macromolecules occurred via the anti- and syn-isomeric diol epoxide metabolites of benzo[ a]pyrene. The majority of adducts in the DNA formed via the anti-diol epoxide (85%), which is similar to that reported for other organisms. However, adduct formation in hemoglobin was via both the anti- and syn-isomeric diol epoxides, which is different from that reported for mice. Furthermore, the amount of adduct formation in both macromolecules depended upon the temperatures at which the fish were maintained. Quantitation of the covalent interaction of genotoxic chemicals such as benzo[ a]pyrene with cellular macromolecules can be a sensitive biological marker of early predictive value in assessing the significance of exposure to human health or ecosystem integrity.
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