Abstract
A simple and convenient method for the simultaneous determination of mono-di- and triacylglycerols has been developed. Acylglycerols from monolaurin to tristearin were separated by reversed-phase high-performance liquid chromatography and detected with the acylglycerol-selective post-column reaction detector. The detector indicated the molar response of all acylglycerols from monolaurin to tristearin. It can, for example, detect 0.07 nmol of 1-monopalmitin and give a linear calibration graph between 0.2 and 121 nmol of 1-monopalmitin. Furthermore, acylglycerols in commercial products could be selectively detected and quantitated without any pretreatment. Therefore, the proposed method should offer a versatile method for the analysis of acylglycerols in natural and commercial products.
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