Abstract
An analytical method of 6-(N-benzylamino)purine (BA) in soil and its persistence in soil have been studied. BA in soil was extracted with a methanol-0.05N sodium hydroxide (3:1) mixture, cleaned up by partition between acidic and neutral aqueous phases and ethyl acetate phase, and determined by gas chromatography with flame ionization or N·P-flame ionization detection. Recoveries from soils fortified with more than 0.1ppm of BA were above 70% and varied somewhat with soil. The lower determination limit was 0.05ppm of BA in soil by flame ionization detection or 0.01ppm by N·P-flame ionization detection. BA dissipation in 2 different kinds of soils was compared by laboratory tests. BA dissipated more rapidly in the flooded condition than in the unflooded condition in both soils. Moreover, dissipation rate varied with soil. A field test showed a typical lag period of BA dissipation. BA dissipation in soil was presumed mainly owing to soil microbiological degradation.
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